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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_179017 |
| Cell Name : | Branching_66R_20052020_46 |
| Archive Name : | Huber_Haapasalo |
| Species Name : | mouse |
| Strain : | C57BL/6J |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 18.0 days
|
| Max Age : | 18.0 days
|
| Gender : |
Male/Female
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| Min Weight : | Not reported
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| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | Not reported |
| Tertiary Cell Class : | Not reported |
| Original Format : | Vaa3D.swc |
| Experiment Protocol : | culture |
| Experimental Condition : | C9-HRE overexpression |
| Staining Method : | ZsGreen1 |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x |
| Reconstruction Method : | Vaa3D |
| Date of Deposition : | 2022-02-14 |
| Date of Upload : | 2022-03-02 |
| Persistence Vector : | Branching_66R_20052020_46.pvec |
| Note : | To express the pathological C9-HRE, a 66 x GGGGCC repeat construct (66R) in pAM/CBA-pl-WPRE-BGH containing inverted repeats of serotype 2 adeno-associated virus (AAV) was used in the transfection. Hippocampal neurons were co-transfected with 66R and ZsGreen1 plasmids at DIV 19 and fixed 48 h after transfection on DIV21. |
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| Reference Article |
| Related Article Reference : | C9orf72 hexanucleotide repeat expansion leads to altered neuronal and dendritic spine morphology and synaptic dysfunction. |
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| Measurements |
| Soma Surface : |
1520.04 μm2 |
| Number of Stems : |
6 |
| Number of Bifurcations : |
3 |
| Number of Branches : |
12 |
| Overall Width : |
171.96 μm |
| Overall Height : |
380.82 μm |
| Overall Depth : |
11.57 μm |
| Average Diameter : |
3.11 μm |
| Total Length : |
1237.06 μm |
| Total Surface : |
10260 μm2 |
| Total Volume : |
12336.4 μm3 |
| Max Euclidean Distance : |
246.46 μm |
| Max Path Distance : |
251.52 μm |
| Max Branch Order : |
2 |
| Average Contraction : |
0.9 |
| Total Fragmentation : |
133 |
| Partition Asymmetry : |
0.33 |
| Average Rall's Ratio : |
1.28 |
| Average Bifurcation Angle Local : |
54.13° |
| Average Bifurcation Angle Remote : |
74.61° |
| Fractal Dimension : |
1.04 |
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