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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_178995 |
| Cell Name : | Branching_66R_20052020_45 |
| Archive Name : | Huber_Haapasalo |
| Species Name : | mouse |
| Strain : | C57BL/6J |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 18.0 days
|
| Max Age : | 18.0 days
|
| Gender : |
Male/Female
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| Min Weight : | Not reported
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| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | Not reported |
| Tertiary Cell Class : | Not reported |
| Original Format : | Vaa3D.swc |
| Experiment Protocol : | culture |
| Experimental Condition : | C9-HRE overexpression |
| Staining Method : | ZsGreen1 |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x |
| Reconstruction Method : | Vaa3D |
| Date of Deposition : | 2022-02-14 |
| Date of Upload : | 2022-03-02 |
| Persistence Vector : | Branching_66R_20052020_45.pvec |
| Note : | To express the pathological C9-HRE, a 66 x GGGGCC repeat construct (66R) in pAM/CBA-pl-WPRE-BGH containing inverted repeats of serotype 2 adeno-associated virus (AAV) was used in the transfection. Hippocampal neurons were co-transfected with 66R and ZsGreen1 plasmids at DIV 19 and fixed 48 h after transfection on DIV21. |
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| Reference Article |
| Related Article Reference : | C9orf72 hexanucleotide repeat expansion leads to altered neuronal and dendritic spine morphology and synaptic dysfunction. |
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| Measurements |
| Soma Surface : |
1808.5 μm2 |
| Number of Stems : |
5 |
| Number of Bifurcations : |
14 |
| Number of Branches : |
33 |
| Overall Width : |
271.51 μm |
| Overall Height : |
500.89 μm |
| Overall Depth : |
8.4 μm |
| Average Diameter : |
2.38 μm |
| Total Length : |
3183.17 μm |
| Total Surface : |
22468.2 μm2 |
| Total Volume : |
17917.3 μm3 |
| Max Euclidean Distance : |
370.57 μm |
| Max Path Distance : |
618.17 μm |
| Max Branch Order : |
6 |
| Average Contraction : |
0.91 |
| Total Fragmentation : |
344 |
| Partition Asymmetry : |
0.59 |
| Average Rall's Ratio : |
1.7 |
| Average Bifurcation Angle Local : |
62.88° |
| Average Bifurcation Angle Remote : |
80.35° |
| Fractal Dimension : |
1.02 |
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