8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_178930
Cell Name :  Branching_2R_20052020_40x_46
Archive Name :  Huber_Haapasalo
Species Name :  mouse
Strain :  C57BL/6J
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  18.0 days
Max Age :  18.0 days
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  embryonic
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  Not reported
Tertiary Cell Class :  Not reported
Original Format :  Vaa3D.swc
Experiment Protocol :  culture
Experimental Condition :  Control
Staining Method :  ZsGreen1
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  40x
Reconstruction Method :  Vaa3D
Date of Deposition :  2022-02-14
Date of Upload :  2022-03-02
Persistence Vector :  Branching_2R_20052020_40x_46.pvec
Note :  As a control, a construct containing 2 x GGGGCC (2R) was used. Hippocampal neurons were co-transfected with 2R and ZsGreen1 plasmids at DIV 19 and fixed 48 h after transfection on DIV21.

Reference Article
Related Article Reference :  C9orf72 hexanucleotide repeat expansion leads to altered neuronal and dendritic spine morphology and synaptic dysfunction.

Measurements
Soma Surface :  1016.51 μm2
Number of Stems :  4
Number of Bifurcations :  9
Number of Branches :  22
Overall Width :  385.78 μm
Overall Height :  462.26 μm
Overall Depth :  21.95 μm
Average Diameter :  2.3 μm
Total Length :  2853.33 μm
Total Surface :  19440 μm2
Total Volume :  13460.1 μm3
Max Euclidean Distance :  351.25 μm
Max Path Distance :  432.46 μm
Max Branch Order :  4
Average Contraction :  0.89
Total Fragmentation :  304
Partition Asymmetry :  0.3
Average Rall's Ratio :  1.64
Average Bifurcation Angle Local :  75.02°
Average Bifurcation Angle Remote :  68.6°
Fractal Dimension :  1.04

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