8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_179032
Cell Name :  Branching_2R_20052020_40x_11
Archive Name :  Huber_Haapasalo
Species Name :  mouse
Strain :  C57BL/6J
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  18.0 days
Max Age :  18.0 days
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  embryonic
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  Not reported
Tertiary Cell Class :  Not reported
Original Format :  Vaa3D.swc
Experiment Protocol :  culture
Experimental Condition :  Control
Staining Method :  ZsGreen1
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  40x
Reconstruction Method :  Vaa3D
Date of Deposition :  2022-02-14
Date of Upload :  2022-03-02
Persistence Vector :  Branching_2R_20052020_40x_11.pvec
Note :  As a control, a construct containing 2 x GGGGCC (2R) was used. Hippocampal neurons were co-transfected with 2R and ZsGreen1 plasmids at DIV 19 and fixed 48 h after transfection on DIV21.

Reference Article
Related Article Reference :  C9orf72 hexanucleotide repeat expansion leads to altered neuronal and dendritic spine morphology and synaptic dysfunction.

Measurements
Soma Surface :  2120.8 μm2
Number of Stems :  4
Number of Bifurcations :  29
Number of Branches :  62
Overall Width :  505.93 μm
Overall Height :  505.8 μm
Overall Depth :  10.88 μm
Average Diameter :  2.37 μm
Total Length :  6031.21 μm
Total Surface :  42273.6 μm2
Total Volume :  30413.3 μm3
Max Euclidean Distance :  374.4 μm
Max Path Distance :  477.03 μm
Max Branch Order :  6
Average Contraction :  0.89
Total Fragmentation :  653
Partition Asymmetry :  0.41
Average Rall's Ratio :  1.68
Average Bifurcation Angle Local :  60.59°
Average Bifurcation Angle Remote :  51.92°
Fractal Dimension :  1.03

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