8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_178238
Cell Name :  Adol_1M_Light-4
Archive Name :  Masachs
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  2.0 months
Max Age :  2.0 months
Gender :  Male
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  hippocampus
Secondary Brain Region :  dentate gyrus
Tertiary Brain Region :  granule layer
Primary Cell Class :  principal cell
Secondary Cell Class :  granule
Tertiary Cell Class :  born at PND28
Original Format :  Neurolucida.dat
Experiment Protocol :  in vivo
Experimental Condition :  Optogenetic stimulation
Staining Method :  enhanced green fluorescent protein, immunostaining
Slicing Direction :  coronal
Slice Thickness :  50  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  100x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-02-16
Date of Upload :  2022-02-23
Persistence Vector :  Adol_1M_Light-4.pvec
Note :  The retrovirus expressing an inhibitory optogene Archaerhodopsin-3 (Arch-EGFP) was injected bilaterally into the dorsal area of the septal DG at postnatal day 28. For Adol-DGNs groups, [AP]: -2.8 mm; [L]:+/-1.5 mm; [P]:-3.5 mm deepness. 2 microliters of viral suspension were injected at a flow rate 0.25 L/min. Illumination was applied throughout the session at 1 Hz pulses of 750 ms. This reconstruction was obtained 1 month after BrdU injection.

Reference Article
Related Article Reference :  The temporal origin of dentate granule neurons dictates their role in spatial memory.

Measurements
Soma Surface :  616.92 μm2
Number of Stems :  1
Number of Bifurcations :  4
Number of Branches :  9
Overall Width :  44.38 μm
Overall Height :  211.1 μm
Overall Depth :  15.86 μm
Average Diameter :  0.07 μm
Total Length :  510.93 μm
Total Surface :  112.36 μm2
Total Volume :  1.97 μm3
Max Euclidean Distance :  231.53 μm
Max Path Distance :  243.78 μm
Max Branch Order :  3
Average Contraction :  0.94
Total Fragmentation :  152
Partition Asymmetry :  0.33
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  55.28°
Average Bifurcation Angle Remote :  21.25°
Fractal Dimension :  1.02

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