8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_250054
Cell Name :  A3_LPS_3
Archive Name :  Garcia-Hernandez_DeSantis
Species Name :  rat
Strain :  Wistar
Structural Domains :  Processes, Soma
Physical Integrity :  Processes Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  2.0 months
Max Age :  3.0 months
Gender :  Male
Min Weight :  300 grams
Max Weight :  400 grams
Development :  adult
Primary Brain Region :  hippocampus
Secondary Brain Region :  dentate gyrus
Tertiary Brain Region :  granule layer
Primary Cell Class :  Glia
Secondary Cell Class :  microglia
Tertiary Cell Class :  Iba1-positive
Original Format :  Neurolucida.dat
Experiment Protocol :  in vivo
Experimental Condition :  lipopolysaccharide injection
Staining Method :  immunostaining
Slicing Direction :  coronal
Slice Thickness :  50  μm
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  20x , 40x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-11-02
Date of Upload :  2022-12-07
Persistence Vector :  A3_LPS_3.pvec
Note :  Glia activation was achieved by intracranial injection in the dorsal hippocampus (coordinates: bregma, -3.8 mm; superior-inferior, 3.0 mm; 2 mm from midline in the left hemisphere) of 2 microliters of saline and LPS at a concentration of 2.5 microgram/microliter. This reconstruction was obtained 8 hours post-injection.

Reference Article
Related Article Reference :  Mapping microglia and astrocyte activation in vivo using diffusion MRI.

Measurements
Soma Surface :  173.56 μm2
Number of Stems :  4
Number of Bifurcations :  11
Number of Branches :  26
Overall Width :  51.44 μm
Overall Height :  15.6 μm
Overall Depth :  3.41 μm
Average Diameter :  0.25 μm
Total Length :  141.83 μm
Total Surface :  111.39 μm2
Total Volume :  6.96 μm3
Max Euclidean Distance :  31.16 μm
Max Path Distance :  34.23 μm
Max Branch Order :  5
Average Contraction :  0.96
Total Fragmentation :  61
Partition Asymmetry :  0.73
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  86.91°
Average Bifurcation Angle Remote :  85.65°
Fractal Dimension :  1.02

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