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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_123501 |
| Cell Name : | A20CGRP_Iba1-Z_124-1 |
| Archive Name : | Morara |
| Species Name : | mouse |
| Strain : | C57BL/6J |
| Structural Domains : | Processes, Soma |
| Physical Integrity : | Processes Moderate |
| Morphological Attributes : | Diameter, 2D, Angles |
| Min Age : | 1.8 months
|
| Max Age : | 2.0 months
|
| Gender : |
Female
|
| Min Weight : | 20 grams
|
| Max Weight : | 23 grams
|
| Development : | young adult |
| Primary Brain Region : | spinal cord |
| Secondary Brain Region : | L6 |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | Glia |
| Secondary Cell Class : | microglia |
| Tertiary Cell Class : | Iba1-positive |
| Original Format : | Neuronstudio.swc |
| Experiment Protocol : | in vitro |
| Experimental Condition : | Experimental autoimmune encephalomyelitis Calcitonin gene-related peptide (CGRP)-treated |
| Staining Method : | immunostaining |
| Slicing Direction : | coronal |
| Slice Thickness : | 10
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 63x |
| Reconstruction Method : | NeuronStudio |
| Date of Deposition : | 2019-08-16 |
| Date of Upload : | 2019-12-13 |
| Persistence Vector : | A20CGRP_Iba1-Z_124-1.pvec |
| Note : | Immunized by subcutaneous injections with 300 ul of 200 ug Myelin Oligodendrocyte Glycoprotein (MOG)35-55 in incomplete Complete Freunds Adjuvant containing 8 mg/ml Mycobacterium tuberculosis. Pertussis toxin (500 ng) injected on day of immunization + two days later (Sardi et al 2014). CGRP osmotically administered (50 pmol/h) by intrathecal catheter. Reconstructions performed at 16 dpi (after 14 days of delivery). |
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| Reference Article |
| Related Article Reference : | Calcitonin gene-related peptide decreases IL-1beta, IL-6 as well as Ym1, Arg1, CD163 expression in a brain tissue context-dependent manner while amelioratingexperimental autoimmune encephalomyelitis. |
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| Measurements |
| Soma Surface : |
4.8 μm2 |
| Number of Stems : |
2 |
| Number of Bifurcations : |
4 |
| Number of Branches : |
10 |
| Overall Width : |
0.84 μm |
| Overall Height : |
15.5 μm |
| Overall Depth : |
4.78 μm |
| Average Diameter : |
0.46 μm |
| Total Length : |
31.56 μm |
| Total Surface : |
51.94 μm2 |
| Total Volume : |
8.97 μm3 |
| Max Euclidean Distance : |
10.31 μm |
| Max Path Distance : |
12.4 μm |
| Max Branch Order : |
2 |
| Average Contraction : |
0.91 |
| Total Fragmentation : |
80 |
| Partition Asymmetry : |
0.5 |
| Average Rall's Ratio : |
0.56 |
| Average Bifurcation Angle Local : |
76.89° |
| Average Bifurcation Angle Remote : |
65.05° |
| Fractal Dimension : |
1.07 |
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