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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_168114 |
| Cell Name : | 9893-day1-isoflurane-SR-T100_a |
| Archive Name : | Suzuki_Dityatev |
| Species Name : | mouse |
| Strain : | CX3CR1-EGFP x C57BL/6J |
| Structural Domains : | Processes, Soma |
| Physical Integrity : | Processes Moderate |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 7.0 months
|
| Max Age : | 8.0 months
|
| Gender : |
Male
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | Glia |
| Secondary Cell Class : | microglia |
| Tertiary Cell Class : | C-X3-C Motif Chemokine Receptor 1-positive |
| Original Format : | Simple Neurite Tracer.swc |
| Experiment Protocol : | in vivo |
| Experimental Condition : | Chronic implant, isoflurane-anesthetized |
| Staining Method : | green fluorescent protein |
| Slicing Direction : | Sagittal |
| Slice Thickness : | Not reported
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | water |
| Magnification : | 20x |
| Reconstruction Method : | Simple Neurite Tracer |
| Date of Deposition : | 2019-07-31 |
| Date of Upload : | 2021-11-16 |
| Persistence Vector : | 9893-day1-isoflurane-SR-T100_a.pvec |
| Note : | This reconstruction was obtained 4 months post-surgery (quadrant 3). After imaging in awake mice, 2-h rest was given to each animal before imaging in the isoflurane-anesthetized condition. The same procedures of imaging as in awake-condition were applied in the other hemisphere, but the animal was head-fixed using a custom-made frame with a heating pad to keep body temperature constant, and anesthetized by 1.5% isoflurane during the imaging, which started 10 min after anesthesia induction. |
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| Reference Article |
| Related Article Reference : | In vivo Two-Photon Imaging of Anesthesia-Specific Alterations in Microglial Surveillance and Photodamage-Directed Motility in Mouse Cortex. |
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| Measurements |
| Soma Surface : |
159.92 μm2 |
| Number of Stems : |
3 |
| Number of Bifurcations : |
13 |
| Number of Branches : |
29 |
| Overall Width : |
56.31 μm |
| Overall Height : |
48.47 μm |
| Overall Depth : |
0.43 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
306.47 μm |
| Total Surface : |
240.7 μm2 |
| Total Volume : |
15.04 μm3 |
| Max Euclidean Distance : |
43.92 μm |
| Max Path Distance : |
51.6 μm |
| Max Branch Order : |
6 |
| Average Contraction : |
0.9 |
| Total Fragmentation : |
221 |
| Partition Asymmetry : |
0.77 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
22.65° |
| Average Bifurcation Angle Remote : |
76.57° |
| Fractal Dimension : |
1.04 |
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