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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_168044 |
| Cell Name : | 9887-day1-awake-SR-SR-T100_a |
| Archive Name : | Suzuki_Dityatev |
| Species Name : | mouse |
| Strain : | CX3CR1-EGFP x C57BL/6J |
| Structural Domains : | Processes, Soma |
| Physical Integrity : | Processes Moderate |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 3.0 months
|
| Max Age : | 4.0 months
|
| Gender : |
Male
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | Glia |
| Secondary Cell Class : | microglia |
| Tertiary Cell Class : | C-X3-C Motif Chemokine Receptor 1-positive |
| Original Format : | Simple Neurite Tracer.swc |
| Experiment Protocol : | in vivo |
| Experimental Condition : | acute implant, awake |
| Staining Method : | green fluorescent protein |
| Slicing Direction : | Sagittal |
| Slice Thickness : | Not reported
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | water |
| Magnification : | 20x |
| Reconstruction Method : | Simple Neurite Tracer |
| Date of Deposition : | 2019-07-31 |
| Date of Upload : | 2021-11-16 |
| Persistence Vector : | 9887-day1-awake-SR-SR-T100_a.pvec |
| Note : | This reconstruction was obtained 1 day after surgery in an awake mouse in quadrant 1. Mice were head-fixed under the two-photon microscope using a Mobile HomeCage device in which animals could freely move a light cage around them. After 10 min habituation, time-lapse imaging was performed for 33 min (100 frames, 20 s interval) by recording z-stacks (6 optical sections with a step size of 2 microns) around 100-150 microns below the pial surface to monitor microglial morphology and dynamics in the resting state. A single cell laser ablation was then achieved by focusing a two-photon laser beam at a single microglia cell in the superficial layer of the cortex to induce a highly precise and reproducible local injury. Immediately after, the same imaging procedure as in the resting state was repeated. |
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| Reference Article |
| Related Article Reference : | In vivo Two-Photon Imaging of Anesthesia-Specific Alterations in Microglial Surveillance and Photodamage-Directed Motility in Mouse Cortex. |
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| Measurements |
| Soma Surface : |
32.75 μm2 |
| Number of Stems : |
2 |
| Number of Bifurcations : |
3 |
| Number of Branches : |
8 |
| Overall Width : |
57.85 μm |
| Overall Height : |
15.15 μm |
| Overall Depth : |
1.13 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
108.07 μm |
| Total Surface : |
84.88 μm2 |
| Total Volume : |
5.3 μm3 |
| Max Euclidean Distance : |
52.68 μm |
| Max Path Distance : |
66.03 μm |
| Max Branch Order : |
2 |
| Average Contraction : |
0.89 |
| Total Fragmentation : |
76 |
| Partition Asymmetry : |
0.33 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
49.85° |
| Average Bifurcation Angle Remote : |
93.84° |
| Fractal Dimension : |
1.05 |
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