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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_143384 |
| Cell Name : | 9-30-15-ppkcd4gfp-uasshepha3x-ypsjm2het-lif_Series032 |
| Archive Name : | Olesnicky |
| Species Name : | drosophila melanogaster |
| Strain : | ppkGal4,UAS-CD4-tdGFP (x) shepBG00836 (x) shepBG00836 (x) yps+/JM2 |
| Structural Domains : | Dendrites, No Soma, No Axon |
| Physical Integrity : | Dendrites Moderate |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | Not reported
|
| Max Age : | Not reported
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| Gender : |
Not reported
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| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | larval |
| Primary Brain Region : | peripheral nervous system |
| Secondary Brain Region : | abdominal |
| Tertiary Brain Region : | segment 4-6 |
| Primary Cell Class : | sensory |
| Secondary Cell Class : | Multidendritic-dendritic arborization (DA) |
| Tertiary Cell Class : | class IV, ddaC |
| Original Format : | NeuronJ.ndf |
| Experiment Protocol : | in vivo |
| Experimental Condition : | Ypsilon schachtel (Yps) mutant (+/-) + shep overexpression |
| Staining Method : | green fluorescent protein, immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x |
| Reconstruction Method : | NeuronJ |
| Date of Deposition : | 2019-11-15 |
| Date of Upload : | 2020-11-05 |
| Persistence Vector : | 9-30-15-ppkcd4gfp-uasshepha3x-ypsjm2het-lif_Series032.pvec |
| Note : | Class IV ddaC neurons were visualized using ppkGal4,UAStdCD4GFP in live, wandering third instar larvae. GAL4/UAS experiments for ddaC neuron dendrite defects were performed at 29 C to increase GAL4 efficiency. Reconstruction was obtained from a Ypsilon schachtel (Yps) mutant heterozygote. For Lexogen reads we inverted the strand flag of the first mate in the BAM files using in-house scripts to account for the reversed strandness of R1 when using the Lexogen SENSE library kit. |
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| Reference Article |
| Related Article Reference : | Shep interacts with posttranscriptional regulators to control dendrite morphogenesis in sensory neurons. |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
1 |
| Number of Bifurcations : |
401 |
| Number of Branches : |
803 |
| Overall Width : |
1064.3 μm |
| Overall Height : |
1004.03 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
21101.3 μm |
| Total Surface : |
16572.9 μm2 |
| Total Volume : |
1035.81 μm3 |
| Max Euclidean Distance : |
1088.71 μm |
| Max Path Distance : |
1915.79 μm |
| Max Branch Order : |
55 |
| Average Contraction : |
0.96 |
| Total Fragmentation : |
4183 |
| Partition Asymmetry : |
0.67 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
103° |
| Average Bifurcation Angle Remote : |
97.42° |
| Fractal Dimension : |
1.01 |
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0
