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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_143369 |
| Cell Name : | 9-17-15-ppkcd4gfpuasshepha3x-trald09277het-29c-lif_Series019 |
| Archive Name : | Olesnicky |
| Species Name : | drosophila melanogaster |
| Strain : | tral+/09277 (x) ppkGal4,UAS-CD4-tdGFP (x) UASpAbp, UASshep3xHA |
| Structural Domains : | Dendrites, No Soma, No Axon |
| Physical Integrity : | Dendrites Moderate |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | Not reported
|
| Max Age : | Not reported
|
| Gender : |
Not reported
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
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| Development : | larval |
| Primary Brain Region : | peripheral nervous system |
| Secondary Brain Region : | abdominal |
| Tertiary Brain Region : | segment 4-6 |
| Primary Cell Class : | sensory |
| Secondary Cell Class : | Multidendritic-dendritic arborization (DA) |
| Tertiary Cell Class : | class IV, ddaC |
| Original Format : | NeuronJ.ndf |
| Experiment Protocol : | in vivo |
| Experimental Condition : | Trailer Hitch (Tral) mutant + Poly(A)-Binding Protein (PABP) + shep/SUP-26 overexpression |
| Staining Method : | green fluorescent protein, immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x |
| Reconstruction Method : | NeuronJ |
| Date of Deposition : | 2019-11-15 |
| Date of Upload : | 2020-11-05 |
| Persistence Vector : | 9-17-15-ppkcd4gfpuasshepha3x-trald09277het-29c-lif_Series019.pvec |
| Note : | Class IV ddaC neurons were visualized using ppkGal4,UAStdCD4GFP in live, wandering third instar larvae. GAL4/UAS experiments for ddaC neuron dendrite defects were performed at 29 C to increase GAL4 efficiency. This reconstruction was obtained from a transheterozygous Tral mutant. Tral mutant was obtained from strong hypomorphic tralKG08052 trald0927 allele and the weak hypomorphic Trald0927. For Lexogen reads we inverted the strand flag of the first mate in the BAM files using in-house scripts to account for the reversed strandness of R1 when using the Lexogen SENSE library kit. |
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| Reference Article |
| Related Article Reference : | Shep interacts with posttranscriptional regulators to control dendrite morphogenesis in sensory neurons. |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
1 |
| Number of Bifurcations : |
301 |
| Number of Branches : |
603 |
| Overall Width : |
1114.57 μm |
| Overall Height : |
1037.39 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
20122.1 μm |
| Total Surface : |
15803.8 μm2 |
| Total Volume : |
987.74 μm3 |
| Max Euclidean Distance : |
1195.5 μm |
| Max Path Distance : |
1726.94 μm |
| Max Branch Order : |
35 |
| Average Contraction : |
0.95 |
| Total Fragmentation : |
3900 |
| Partition Asymmetry : |
0.63 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
102.96° |
| Average Bifurcation Angle Remote : |
96.76° |
| Fractal Dimension : |
1.01 |
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0
