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Details about selected cell |
NeuroMorpho.Org ID : | NMO_168133 |
Cell Name : | 8979-day1-isoflurane-SR-SR-T100_c |
Archive Name : | Suzuki_Dityatev |
Species Name : | mouse |
Strain : | CX3CR1-EGFP x C57BL/6J |
Structural Domains : | Processes, Soma |
Physical Integrity : | Processes Moderate |
Morphological Attributes : | No Diameter, 3D, Angles |
Min Age : | 3.0 months
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Max Age : | 4.0 months
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Gender : |
Male
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Min Weight : | Not reported
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Max Weight : | Not reported
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Development : | adult |
Primary Brain Region : | neocortex |
Secondary Brain Region : | Not reported |
Tertiary Brain Region : | Not reported |
Primary Cell Class : | Glia |
Secondary Cell Class : | microglia |
Tertiary Cell Class : | C-X3-C Motif Chemokine Receptor 1-positive |
Original Format : | Simple Neurite Tracer.swc |
Experiment Protocol : | in vivo |
Experimental Condition : | acute implant, isoflurane-anesthetized |
Staining Method : | green fluorescent protein |
Slicing Direction : | Sagittal |
Slice Thickness : | Not reported
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Tissue Shrinkage : |
Not reported
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Objective Type : | water |
Magnification : | 20x |
Reconstruction Method : | Simple Neurite Tracer |
Date of Deposition : | 2019-07-31 |
Date of Upload : | 2021-11-16 |
Persistence Vector : | 8979-day1-isoflurane-SR-SR-T100_c.pvec |
Note : | This reconstruction was obtained 1 day after surgery (quadrant 3). After imaging in awake mice, 2-h rest was given to each animal before imaging in the isoflurane-anesthetized condition. The same procedures of imaging as in awake-condition were applied in the other hemisphere, but the animal was head-fixed using a custom-made frame with a heating pad to keep body temperature constant, and anesthetized by 1.5% isoflurane during the imaging, which started 10 min after anesthesia induction. |
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Reference Article |
Related Article Reference : | In vivo Two-Photon Imaging of Anesthesia-Specific Alterations in Microglial Surveillance and Photodamage-Directed Motility in Mouse Cortex. |
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Measurements |
Soma Surface : |
126.22 μm2 |
Number of Stems : |
4 |
Number of Bifurcations : |
11 |
Number of Branches : |
26 |
Overall Width : |
50.18 μm |
Overall Height : |
32.35 μm |
Overall Depth : |
3.5 μm |
Average Diameter : |
0.25 μm |
Total Length : |
226.42 μm |
Total Surface : |
177.83 μm2 |
Total Volume : |
11.11 μm3 |
Max Euclidean Distance : |
29.68 μm |
Max Path Distance : |
40.71 μm |
Max Branch Order : |
5 |
Average Contraction : |
0.89 |
Total Fragmentation : |
160 |
Partition Asymmetry : |
0.45 |
Average Rall's Ratio : |
2 |
Average Bifurcation Angle Local : |
58.31° |
Average Bifurcation Angle Remote : |
94.73° |
Fractal Dimension : |
1.04 |
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