8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_168133
Cell Name :  8979-day1-isoflurane-SR-SR-T100_c
Archive Name :  Suzuki_Dityatev
Species Name :  mouse
Strain :  CX3CR1-EGFP x C57BL/6J
Structural Domains :  Processes, Soma
Physical Integrity :  Processes Moderate
Morphological Attributes :  No Diameter, 3D, Angles
Min Age :  3.0 months
Max Age :  4.0 months
Gender :  Male
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  Glia
Secondary Cell Class :  microglia
Tertiary Cell Class :  C-X3-C Motif Chemokine Receptor 1-positive
Original Format :  Simple Neurite Tracer.swc
Experiment Protocol :  in vivo
Experimental Condition :  acute implant, isoflurane-anesthetized
Staining Method :  green fluorescent protein
Slicing Direction :  Sagittal
Slice Thickness :  Not reported
Tissue Shrinkage :  Not reported
Objective Type :  water
Magnification :  20x
Reconstruction Method :  Simple Neurite Tracer
Date of Deposition :  2019-07-31
Date of Upload :  2021-11-16
Persistence Vector :  8979-day1-isoflurane-SR-SR-T100_c.pvec
Note :  This reconstruction was obtained 1 day after surgery (quadrant 3). After imaging in awake mice, 2-h rest was given to each animal before imaging in the isoflurane-anesthetized condition. The same procedures of imaging as in awake-condition were applied in the other hemisphere, but the animal was head-fixed using a custom-made frame with a heating pad to keep body temperature constant, and anesthetized by 1.5% isoflurane during the imaging, which started 10 min after anesthesia induction.

Reference Article
Related Article Reference :  In vivo Two-Photon Imaging of Anesthesia-Specific Alterations in Microglial Surveillance and Photodamage-Directed Motility in Mouse Cortex.

Measurements
Soma Surface :  126.22 μm2
Number of Stems :  4
Number of Bifurcations :  11
Number of Branches :  26
Overall Width :  50.18 μm
Overall Height :  32.35 μm
Overall Depth :  3.5 μm
Average Diameter :  0.25 μm
Total Length :  226.42 μm
Total Surface :  177.83 μm2
Total Volume :  11.11 μm3
Max Euclidean Distance :  29.68 μm
Max Path Distance :  40.71 μm
Max Branch Order :  5
Average Contraction :  0.89
Total Fragmentation :  160
Partition Asymmetry :  0.45
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  58.31°
Average Bifurcation Angle Remote :  94.73°
Fractal Dimension :  1.04

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