8.6.79 - Released: 2025-04-16 - Content: 273841 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_168175
Cell Name :  7828-day2-ketamine-SR-SR-T100_a
Archive Name :  Suzuki_Dityatev
Species Name :  mouse
Strain :  CX3CR1-EGFP x C57BL/6J
Structural Domains :  Processes, Soma
Physical Integrity :  Processes Moderate
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  3.0 months
Max Age :  4.0 months
Gender :  Male
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  Glia
Secondary Cell Class :  microglia
Tertiary Cell Class :  C-X3-C Motif Chemokine Receptor 1-positive
Original Format :  Simple Neurite Tracer.swc
Experiment Protocol :  in vivo
Experimental Condition :  acute implant, ketamine-anesthetized
Staining Method :  green fluorescent protein
Slicing Direction :  Sagittal
Slice Thickness :  Not reported
Tissue Shrinkage :  Not reported
Objective Type :  water
Magnification :  20x
Reconstruction Method :  Simple Neurite Tracer
Date of Deposition :  2019-07-31
Date of Upload :  2021-11-16
Persistence Vector :  7828-day2-ketamine-SR-SR-T100_a.pvec
Note :  This reconstruction was obtained 2 days after surgery (quadrant 4). After 2-h rest, animals were intraperitoneally injected with ketamine (90 mg/kg body weight) and xylazine (18 mg/kg body weight) in 0.9% NaCl solution and placed in a head fixation frame in the same manner as used for imaging in isoflurane-anesthetized condition.

Reference Article
Related Article Reference :  In vivo Two-Photon Imaging of Anesthesia-Specific Alterations in Microglial Surveillance and Photodamage-Directed Motility in Mouse Cortex.

Measurements
Soma Surface :  211.05 μm2
Number of Stems :  4
Number of Bifurcations :  8
Number of Branches :  20
Overall Width :  34.64 μm
Overall Height :  24.93 μm
Overall Depth :  2.05 μm
Average Diameter :  0.25 μm
Total Length :  154.36 μm
Total Surface :  121.24 μm2
Total Volume :  7.58 μm3
Max Euclidean Distance :  23.87 μm
Max Path Distance :  27.66 μm
Max Branch Order :  3
Average Contraction :  0.91
Total Fragmentation :  109
Partition Asymmetry :  0.5
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  55.53°
Average Bifurcation Angle Remote :  73.1°
Fractal Dimension :  1.03

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