8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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Details about selected cell
NeuroMorpho.Org ID :  NMO_181937
Cell Name :  7-MAX_KO-GLUTA-MOCK-555-MAP2-EGFP-63X
Archive Name :  Siqueira_Guil
Species Name :  human
Strain :  Not applicable
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  2.5 months
Max Age :  2.5 months
Gender :  Not reported
Min Weight :  Not reported
Max Weight :  Not reported
Development :  fetus
Primary Brain Region :  mesencephalon
Secondary Brain Region :  ventral
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  glutamatergic
Tertiary Cell Class :  neural progenitor cell derived
Original Format :  Neuronstudio.swc
Experiment Protocol :  culture
Experimental Condition :  Mecp2 KO
Staining Method :  Farnesylated enhanced green fluorescent protein (eGFP-F)
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  63x
Reconstruction Method :  NeuronStudio
Date of Deposition :  2022-02-07
Date of Upload :  2022-04-06
Persistence Vector :  7-MAX_KO-GLUTA-MOCK-555-MAP2-EGFP-63X.pvec
Note :  CRISPR-Cas9 technology was used for engineering MECP2 KO in human ReNCells VM. Mecp2 KO was confirmed by western blotting.

Reference Article
Related Article Reference :  Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome.

Measurements
Soma Surface :  2419.1 μm2
Number of Stems :  5
Number of Bifurcations :  9
Number of Branches :  23
Overall Width :  97.44 μm
Overall Height :  218.37 μm
Overall Depth :  0 μm
Average Diameter :  2.53 μm
Total Length :  520.72 μm
Total Surface :  8151.5 μm2
Total Volume :  16066.8 μm3
Max Euclidean Distance :  123.62 μm
Max Path Distance :  126.76 μm
Max Branch Order :  3
Average Contraction :  0.97
Total Fragmentation :  245
Partition Asymmetry :  0.56
Average Rall's Ratio :  0.88
Average Bifurcation Angle Local :  88.07°
Average Bifurcation Angle Remote :  81.7°
Fractal Dimension :  1.02

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