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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_146720 |
| Cell Name : | 5mOrange_final-3 |
| Archive Name : | Firestein |
| Species Name : | rat |
| Strain : | Sprague-Dawley |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 18.0 days
|
| Max Age : | 18.0 days
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | CA3 |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | pyramidal |
| Tertiary Cell Class : | Developing |
| Original Format : | NeuronJ.swc |
| Experiment Protocol : | culture |
| Experimental Condition : | Carboxypeptidase E (CPE) knockdown |
| Staining Method : | enhanced green fluorescent protein, immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x |
| Reconstruction Method : | NeuronJ |
| Date of Deposition : | 2020-11-16 |
| Date of Upload : | 2021-01-05 |
| Persistence Vector : | 5mOrange_final-3.pvec |
| Note : | Cotransfected with pCAG-mOrange and short-hairpin (sh)RNA against CPE at DIV 7 using Lipofectamine LTX with Plus, fixed at DIV 10 with 4% PFA in PBS, and immunostained for GFP and MAP2. |
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|
| Reference Article |
| Related Article Reference : | Cortical Neuron Migration and Dendrite Morphology are Regulated by Carboxypeptidase E. |
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| Measurements |
| Soma Surface : |
13.04 μm2 |
| Number of Stems : |
12 |
| Number of Bifurcations : |
8 |
| Number of Branches : |
28 |
| Overall Width : |
15.68 μm |
| Overall Height : |
20.18 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.3 μm |
| Total Length : |
107.87 μm |
| Total Surface : |
101.67 μm2 |
| Total Volume : |
7.63 μm3 |
| Max Euclidean Distance : |
13.78 μm |
| Max Path Distance : |
15.18 μm |
| Max Branch Order : |
3 |
| Average Contraction : |
0.97 |
| Total Fragmentation : |
211 |
| Partition Asymmetry : |
0.62 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
45.4° |
| Average Bifurcation Angle Remote : |
44.2° |
| Fractal Dimension : |
1.01 |
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