8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_243915
Cell Name :  40xcv3n60_2
Archive Name :  Guizzetti
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Neurites, Soma
Physical Integrity :  Neurites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  1.0 day
Max Age :  1.0 day
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  neonatal
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Not reported
Original Format :  Neurolucida.dat
Experiment Protocol :  culture
Experimental Condition :  rtPA
Staining Method :  immunostaining
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  40x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-05-05
Date of Upload :  2022-11-07
Persistence Vector :  40xcv3n60_2.pvec
Note :  Three days after sub-culturing astrocytes in the appropriate vessels, medium was changed to DMEM and replaced 24 h later with fresh DMEM/0.1% BSA/penstrep medium containing 50 nM of active recombinant rat tPA for 16h.

Reference Article
Related Article Reference :  Astrocyte tissue plasminogen activator expression during brain development and its role in pyramidal neuron neurite outgrowth.

Measurements
Soma Surface :  3765.85 μm2
Number of Stems :  5
Number of Bifurcations :  4
Number of Branches :  13
Overall Width :  448.06 μm
Overall Height :  143.64 μm
Overall Depth :  0 μm
Average Diameter :  3.53 μm
Total Length :  1372.4 μm
Total Surface :  15219.7 μm2
Total Volume :  13431.3 μm3
Max Euclidean Distance :  336.96 μm
Max Path Distance :  372.12 μm
Max Branch Order :  2
Average Contraction :  0.96
Total Fragmentation :  56
Partition Asymmetry :  0.25
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  100.19°
Average Bifurcation Angle Remote :  73.03°
Fractal Dimension :  1.01

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