8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_246546
Cell Name :  40xcv2n36_2
Archive Name :  Guizzetti
Species Name :  rat
Strain :  Sprague-Dawley
Structural Domains :  Neurites, Soma
Physical Integrity :  Neurites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  1.0 day
Max Age :  1.0 day
Gender :  Male/Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  neonatal
Primary Brain Region :  hippocampus
Secondary Brain Region :  Not reported
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Not reported
Original Format :  Neurolucida.dat
Experiment Protocol :  culture
Experimental Condition :  rtPA
Staining Method :  immunostaining
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  dry
Magnification :  40x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-05-05
Date of Upload :  2022-11-07
Persistence Vector :  40xcv2n36_2.pvec
Note :  Three days after sub-culturing astrocytes in the appropriate vessels, medium was changed to DMEM and replaced 24 h later with fresh DMEM/0.1% BSA/penstrep medium containing 50 nM of active recombinant rat tPA for 16h.

Reference Article
Related Article Reference :  Astrocyte tissue plasminogen activator expression during brain development and its role in pyramidal neuron neurite outgrowth.

Measurements
Soma Surface :  3940.19 μm2
Number of Stems :  4
Number of Bifurcations :  1
Number of Branches :  6
Overall Width :  623.71 μm
Overall Height :  157.37 μm
Overall Depth :  0 μm
Average Diameter :  4.54 μm
Total Length :  992.5 μm
Total Surface :  14155.9 μm2
Total Volume :  16066.9 μm3
Max Euclidean Distance :  550.96 μm
Max Path Distance :  601.42 μm
Max Branch Order :  1
Average Contraction :  0.95
Total Fragmentation :  45
Partition Asymmetry :  0
Average Rall's Ratio :  2
Average Bifurcation Angle Local :  64.14°
Average Bifurcation Angle Remote :  94.85°
Fractal Dimension :  1.01

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