8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_182007
Cell Name :  4-WT-GLUTA-478-555-MAP2-EGFP-63X
Archive Name :  Siqueira_Guil
Species Name :  human
Strain :  Not applicable
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  2.5 months
Max Age :  2.5 months
Gender :  Not reported
Min Weight :  Not reported
Max Weight :  Not reported
Development :  fetus
Primary Brain Region :  mesencephalon
Secondary Brain Region :  ventral
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  glutamatergic
Tertiary Cell Class :  neural progenitor cell derived
Original Format :  Neuronstudio.swc
Experiment Protocol :  culture
Experimental Condition :  uc478 overexpression
Staining Method :  Farnesylated enhanced green fluorescent protein (eGFP-F)
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  63x
Reconstruction Method :  NeuronStudio
Date of Deposition :  2022-02-07
Date of Upload :  2022-04-06
Persistence Vector :  4-WT-GLUTA-478-555-MAP2-EGFP-63X.pvec
Note :  To improve circularization, a sequence of 35 nt upstream and downstream of introns was added. uc.478 were overexpressed by cloning into the BamHI site of the vector pLVX-shRNA2.

Reference Article
Related Article Reference :  Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome.

Measurements
Soma Surface :  130.15 μm2
Number of Stems :  1
Number of Bifurcations :  1
Number of Branches :  3
Overall Width :  4.97 μm
Overall Height :  21.05 μm
Overall Depth :  0 μm
Average Diameter :  1.6 μm
Total Length :  28.25 μm
Total Surface :  176.63 μm2
Total Volume :  107.78 μm3
Max Euclidean Distance :  22.54 μm
Max Path Distance :  22.8 μm
Max Branch Order :  1
Average Contraction :  0.98
Total Fragmentation :  22
Partition Asymmetry :  0
Average Rall's Ratio :  0.76
Average Bifurcation Angle Local :  75.45°
Average Bifurcation Angle Remote :  61.94°
Fractal Dimension :  1.01

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