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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_148668 |
| Cell Name : | 4--TEE-vDG |
| Archive Name : | Wang_XD |
| Species Name : | mouse |
| Strain : | C57BL/6N |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | Diameter, 3D, Angles |
| Min Age : | 4.0 months
|
| Max Age : | 2.0 months
|
| Gender : |
Male
|
| Min Weight : | 25 grams
|
| Max Weight : | 25 grams
|
| Development : | adult |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | dentate gyrus |
| Tertiary Brain Region : | ventral, granule layer |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | granule |
| Tertiary Cell Class : | glutamatergic, Doublecortin (DCX)-containing, adult-born |
| Original Format : | Neuronstudio.swc |
| Experiment Protocol : | in vivo |
| Experimental Condition : | tactile experience enrichment (TEE) |
| Staining Method : | enhanced green fluorescent protein |
| Slicing Direction : | coronal |
| Slice Thickness : | 120
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 40x |
| Reconstruction Method : | NeuronStudio |
| Date of Deposition : | 2021-01-11 |
| Date of Upload : | 2021-01-15 |
| Persistence Vector : | 4--TEE-vDG.pvec |
| Note : | To label adult-born dentate granule cells, engineered self-inactivating murine retrovirus (RV) expressing enhanced green fluorescent protein (EGFP) under the control of the Ubi promoter was produced and purified. RV solution (1 microl per injection site) was bilaterally delivered to infect both dDG and vDG neurons (2.5mm posterior to bregma, 2.5 mm lateral from midline, and 1.8 mm ventral from dura). After viral microinjection, the micropipette was left in site for 5-7 min. |
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| Reference Article |
| Related Article Reference : | Tactile modulation of memory and anxiety requires dentate granule cells along the dorsoventral axis. |
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| Measurements |
| Soma Surface : |
102.6 μm2 |
| Number of Stems : |
1 |
| Number of Bifurcations : |
11 |
| Number of Branches : |
23 |
| Overall Width : |
143.89 μm |
| Overall Height : |
172.93 μm |
| Overall Depth : |
60 μm |
| Average Diameter : |
1.39 μm |
| Total Length : |
1343.62 μm |
| Total Surface : |
5806.34 μm2 |
| Total Volume : |
2107.98 μm3 |
| Max Euclidean Distance : |
195.76 μm |
| Max Path Distance : |
218.47 μm |
| Max Branch Order : |
4 |
| Average Contraction : |
0.91 |
| Total Fragmentation : |
758 |
| Partition Asymmetry : |
0.23 |
| Average Rall's Ratio : |
1.53 |
| Average Bifurcation Angle Local : |
51° |
| Average Bifurcation Angle Remote : |
32.69° |
| Fractal Dimension : |
1.03 |
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