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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_254874 |
| Cell Name : | 296L1 |
| Archive Name : | Morgan |
| Species Name : | mouse |
| Strain : | Rosa26-LSL-Bdnf-myc-IG/Pf4iCre |
| Structural Domains : | Dendrites, No Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 2.0 months
|
| Max Age : | 6.0 months
|
| Gender : |
Male/Female
|
| Min Weight : | 20 grams
|
| Max Weight : | 28 grams
|
| Development : | adult |
| Primary Brain Region : | retina |
| Secondary Brain Region : | left |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | ganglion |
| Tertiary Cell Class : | Not reported |
| Original Format : | Imaris.ims |
| Experiment Protocol : | in vivo |
| Experimental Condition : | optic nerve crush (ONC) |
| Staining Method : | DiOlistics |
| Slicing Direction : | flattened |
| Slice Thickness : | 1.67
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x |
| Reconstruction Method : | Imaris |
| Date of Deposition : | 2022-11-09 |
| Date of Upload : | 2023-02-27 |
| Persistence Vector : | 296L1.pvec |
| Note : | The nerve was crushed 2mm posteriorly to the globe for 5 seconds using the self-closing action of Dumont N5 cross-action forceps to provide a consistent compression force across procedures. Following the nerve crush, tissues were returned to their original position and Viscotears and a cover slip were applied to the cornea to allow direct visualisation of the optic nerve head and vasculature. After 7 days, all mice were culled by cervical dislocation and the retinas dissected as previously described and prepared for DiOlistic labelling. |
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| Reference Article |
| Related Article Reference : | BDDF released from blood platelets prevents dendritic atrophy of lesioned adult CNS neurons |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
2 |
| Number of Bifurcations : |
106 |
| Number of Branches : |
214 |
| Overall Width : |
80.16 μm |
| Overall Height : |
133.07 μm |
| Overall Depth : |
7.45 μm |
| Average Diameter : |
1.28 μm |
| Total Length : |
1747.75 μm |
| Total Surface : |
7036.94 μm2 |
| Total Volume : |
2273.61 μm3 |
| Max Euclidean Distance : |
108.93 μm |
| Max Path Distance : |
172.95 μm |
| Max Branch Order : |
27 |
| Average Contraction : |
0.9 |
| Total Fragmentation : |
2845 |
| Partition Asymmetry : |
0.6 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
79.39° |
| Average Bifurcation Angle Remote : |
81.43° |
| Fractal Dimension : |
1.04 |
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