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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_146447 |
| Cell Name : | 28mOrange_final-1 |
| Archive Name : | Firestein |
| Species Name : | rat |
| Strain : | Sprague-Dawley |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 18.0 days
|
| Max Age : | 18.0 days
|
| Gender : |
Male/Female
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| Min Weight : | Not reported
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| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | hippocampus |
| Secondary Brain Region : | CA3 |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | pyramidal |
| Tertiary Cell Class : | Developing |
| Original Format : | NeuronJ.swc |
| Experiment Protocol : | culture |
| Experimental Condition : | Carboxypeptidase E (CPE) overexpression |
| Staining Method : | enhanced green fluorescent protein, immunostaining |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x |
| Reconstruction Method : | NeuronJ |
| Date of Deposition : | 2020-11-16 |
| Date of Upload : | 2021-01-05 |
| Persistence Vector : | 28mOrange_final-1.pvec |
| Note : | Cotransfected with pCAG-mOrange and pEGFP-C1-CPE-C10 at DIV 7 using Lipofectamine LTX with Plus, fixed at DIV 10 with 4% PFA in PBS, and immunostained for GFP and MAP2. The SWC units for this cell are expressed in pixels rather than in microns. Thus, the morphometric measurements can be compared within other cells within this archive, but not across the rest of the database. |
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| Reference Article |
| Related Article Reference : | Cortical Neuron Migration and Dendrite Morphology are Regulated by Carboxypeptidase E. |
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| Measurements |
| Soma Surface : |
17.94 μm2 |
| Number of Stems : |
10 |
| Number of Bifurcations : |
13 |
| Number of Branches : |
36 |
| Overall Width : |
22.04 μm |
| Overall Height : |
33.4 μm |
| Overall Depth : |
0 μm |
| Average Diameter : |
0.3 μm |
| Total Length : |
166.01 μm |
| Total Surface : |
156.46 μm2 |
| Total Volume : |
11.73 μm3 |
| Max Euclidean Distance : |
33.67 μm |
| Max Path Distance : |
37.82 μm |
| Max Branch Order : |
5 |
| Average Contraction : |
0.97 |
| Total Fragmentation : |
312 |
| Partition Asymmetry : |
0.43 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
53.5° |
| Average Bifurcation Angle Remote : |
55.07° |
| Fractal Dimension : |
1.01 |
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