8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_182070
Cell Name :  27Aug18_YL_S1_Slice2_cellA
Archive Name :  Keller
Species Name :  mouse
Strain :  C57BL/6
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  Diameter, 3D, Angles
Min Age :  40.0 days
Max Age :  45.0 days
Gender :  Male
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  somatosensory
Tertiary Brain Region :  layer 5
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Spiny
Original Format :  Neurolucida.dat
Experiment Protocol :  in vitro
Experimental Condition :  Perinatal Fentanyl Exposure
Staining Method :  Biocytin, Streptavidin-Cy3
Slicing Direction :  coronal
Slice Thickness :  300  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  20x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-03-02
Date of Upload :  2022-04-12
Persistence Vector :  27Aug18_YL_S1_Slice2_cellA.pvec
Note :  When copulatory plugs were identified, we removed the sires, and added fentanyl to the water hydration pouches. We used 1, 10, or 100 mg/ml fentanyl citrate (calculated as free base) in 2% (w/v) saccharin in the water hydration pouches, which were replenished weekly until litters were weaned.

Reference Article
Related Article Reference :  Perinatal Fentanyl Exposure Leads to Long-Lasting Impairments in Somatosensory Circuit Function and Behavior.

Measurements
Soma Surface :  607.13 μm2
Number of Stems :  6
Number of Bifurcations :  18
Number of Branches :  42
Overall Width :  198.69 μm
Overall Height :  258.87 μm
Overall Depth :  37.5 μm
Average Diameter :  0.74 μm
Total Length :  2707.94 μm
Total Surface :  6002.74 μm2
Total Volume :  1129.3 μm3
Max Euclidean Distance :  173.64 μm
Max Path Distance :  240.45 μm
Max Branch Order :  4
Average Contraction :  0.9
Total Fragmentation :  615
Partition Asymmetry :  0.38
Average Rall's Ratio :  1.53
Average Bifurcation Angle Local :  79.53°
Average Bifurcation Angle Remote :  44.05°
Fractal Dimension :  1.03

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