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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_182069 |
| Cell Name : | 27Aug18_YL_S1_Slice1_cellA |
| Archive Name : | Keller |
| Species Name : | mouse |
| Strain : | C57BL/6 |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | Diameter, 3D, Angles |
| Min Age : | 40.0 days
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| Max Age : | 45.0 days
|
| Gender : |
Male
|
| Min Weight : | Not reported
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| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | somatosensory |
| Tertiary Brain Region : | layer 5 |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | pyramidal |
| Tertiary Cell Class : | Spiny |
| Original Format : | Neurolucida.dat |
| Experiment Protocol : | in vitro |
| Experimental Condition : | Perinatal Fentanyl Exposure |
| Staining Method : | Biocytin, Streptavidin-Cy3 |
| Slicing Direction : | coronal |
| Slice Thickness : | 300
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 20x |
| Reconstruction Method : | Neurolucida |
| Date of Deposition : | 2022-03-02 |
| Date of Upload : | 2022-04-12 |
| Persistence Vector : | 27Aug18_YL_S1_Slice1_cellA.pvec |
| Note : | When copulatory plugs were identified, we removed the sires, and added fentanyl to the water hydration pouches. We used 1, 10, or 100 mg/ml fentanyl citrate (calculated as free base) in 2% (w/v) saccharin in the water hydration pouches, which were replenished weekly until litters were weaned. |
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| Reference Article |
| Related Article Reference : | Perinatal Fentanyl Exposure Leads to Long-Lasting Impairments in Somatosensory Circuit Function and Behavior. |
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| Measurements |
| Soma Surface : |
301.8 μm2 |
| Number of Stems : |
5 |
| Number of Bifurcations : |
35 |
| Number of Branches : |
75 |
| Overall Width : |
160.41 μm |
| Overall Height : |
167.11 μm |
| Overall Depth : |
57.99 μm |
| Average Diameter : |
0.95 μm |
| Total Length : |
2168.75 μm |
| Total Surface : |
6326.94 μm2 |
| Total Volume : |
1547.44 μm3 |
| Max Euclidean Distance : |
121.72 μm |
| Max Path Distance : |
174.88 μm |
| Max Branch Order : |
5 |
| Average Contraction : |
0.89 |
| Total Fragmentation : |
631 |
| Partition Asymmetry : |
0.34 |
| Average Rall's Ratio : |
1.84 |
| Average Bifurcation Angle Local : |
70.47° |
| Average Bifurcation Angle Remote : |
69.22° |
| Fractal Dimension : |
1.04 |
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