8.6.92 - Released: 2025-10-03 - Content: 280015 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_182069
Cell Name :  27Aug18_YL_S1_Slice1_cellA
Archive Name :  Keller
Species Name :  mouse
Strain :  C57BL/6
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  Diameter, 3D, Angles
Min Age :  40.0 days
Max Age :  45.0 days
Gender :  Male
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  somatosensory
Tertiary Brain Region :  layer 5
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Spiny
Original Format :  Neurolucida.dat
Experiment Protocol :  in vitro
Experimental Condition :  Perinatal Fentanyl Exposure
Staining Method :  Biocytin, Streptavidin-Cy3
Slicing Direction :  coronal
Slice Thickness :  300  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  20x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-03-02
Date of Upload :  2022-04-12
Persistence Vector :  27Aug18_YL_S1_Slice1_cellA.pvec
Note :  When copulatory plugs were identified, we removed the sires, and added fentanyl to the water hydration pouches. We used 1, 10, or 100 mg/ml fentanyl citrate (calculated as free base) in 2% (w/v) saccharin in the water hydration pouches, which were replenished weekly until litters were weaned.

Reference Article
Related Article Reference :  Perinatal Fentanyl Exposure Leads to Long-Lasting Impairments in Somatosensory Circuit Function and Behavior.

Measurements
Soma Surface :  301.8 μm2
Number of Stems :  5
Number of Bifurcations :  35
Number of Branches :  75
Overall Width :  160.41 μm
Overall Height :  167.11 μm
Overall Depth :  57.99 μm
Average Diameter :  0.95 μm
Total Length :  2168.75 μm
Total Surface :  6326.94 μm2
Total Volume :  1547.44 μm3
Max Euclidean Distance :  121.72 μm
Max Path Distance :  174.88 μm
Max Branch Order :  5
Average Contraction :  0.89
Total Fragmentation :  631
Partition Asymmetry :  0.34
Average Rall's Ratio :  1.84
Average Bifurcation Angle Local :  70.47°
Average Bifurcation Angle Remote :  69.22°
Fractal Dimension :  1.04

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