8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_182081
Cell Name :  26Apr19_JA_S1_slice2_cellA
Archive Name :  Keller
Species Name :  mouse
Strain :  C57BL/6
Structural Domains :  Dendrites, Soma, Axon
Physical Integrity :  Dendrites Complete, Axon Moderate
Morphological Attributes :  Diameter, 3D, Angles
Min Age :  40.0 days
Max Age :  45.0 days
Gender :  Female
Min Weight :  Not reported
Max Weight :  Not reported
Development :  adult
Primary Brain Region :  neocortex
Secondary Brain Region :  somatosensory
Tertiary Brain Region :  layer 5
Primary Cell Class :  principal cell
Secondary Cell Class :  pyramidal
Tertiary Cell Class :  Spiny
Original Format :  Neurolucida.dat
Experiment Protocol :  in vitro
Experimental Condition :  Perinatal Fentanyl Exposure
Staining Method :  Biocytin, Streptavidin-Cy3
Slicing Direction :  coronal
Slice Thickness :  300  μm
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  20x
Reconstruction Method :  Neurolucida
Date of Deposition :  2022-03-02
Date of Upload :  2022-04-12
Persistence Vector :  26Apr19_JA_S1_slice2_cellA.pvec
Note :  When copulatory plugs were identified, we removed the sires, and added fentanyl to the water hydration pouches. We used 1, 10, or 100 mg/ml fentanyl citrate (calculated as free base) in 2% (w/v) saccharin in the water hydration pouches, which were replenished weekly until litters were weaned.

Reference Article
Related Article Reference :  Perinatal Fentanyl Exposure Leads to Long-Lasting Impairments in Somatosensory Circuit Function and Behavior.

Measurements
Soma Surface :  424.57 μm2
Number of Stems :  8
Number of Bifurcations :  19
Number of Branches :  46
Overall Width :  221.33 μm
Overall Height :  467.3 μm
Overall Depth :  67.47 μm
Average Diameter :  1.23 μm
Total Length :  2664.98 μm
Total Surface :  9971.41 μm2
Total Volume :  3144.93 μm3
Max Euclidean Distance :  368.8 μm
Max Path Distance :  375.42 μm
Max Branch Order :  5
Average Contraction :  0.92
Total Fragmentation :  558
Partition Asymmetry :  0.24
Average Rall's Ratio :  1.55
Average Bifurcation Angle Local :  64.88°
Average Bifurcation Angle Remote :  67.56°
Fractal Dimension :  1.02

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