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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_177696 |
| Cell Name : | 20_slit1 |
| Archive Name : | VanDerZee |
| Species Name : | mouse |
| Strain : | C57BL/6J |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 2.0 months
|
| Max Age : | 2.0 months
|
| Gender : |
Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | medial prefrontal |
| Tertiary Brain Region : | layer 2-3, ventral |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | pyramidal |
| Tertiary Cell Class : | Not reported |
| Original Format : | Neurolucida.dat |
| Experiment Protocol : | in vitro |
| Experimental Condition : | Slit1 knockdown |
| Staining Method : | green fluorescent protein |
| Slicing Direction : | coronal |
| Slice Thickness : | 100
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x |
| Reconstruction Method : | Neurolucida |
| Date of Deposition : | 2022-01-11 |
| Date of Upload : | 2022-02-16 |
| Persistence Vector : | 20_slit1.pvec |
| Note : | Slit1 KD in the vmPFC was accomplished by injecting HSV vectors expressing a miRNA targeting Slit1, as well as green fluorescent protein (GFP) (miR-Slit1). For viral delivery, the following stereotaxic coordinates were applied: +1.8 mm (anterior/posterior), +0.75 mm (medial/lateral), -2.7 mm (dorsal/ventral) at an angle of 15 degrees from the midline (relative to Bregma). A total of 0.5 microliters of HSV solution was delivered bilaterally over a 5-minute period (0.1microlitres/min), followed by 5 minutes of rest after which the needle was slowly removed. |
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| Reference Article |
| Related Article Reference : | Sex-Specific Role for SLIT1 in Regulating Stress Susceptibility. |
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| Measurements |
| Soma Surface : |
672.09 μm2 |
| Number of Stems : |
4 |
| Number of Bifurcations : |
5 |
| Number of Branches : |
14 |
| Overall Width : |
189.01 μm |
| Overall Height : |
307.6 μm |
| Overall Depth : |
21.28 μm |
| Average Diameter : |
0.19 μm |
| Total Length : |
997.89 μm |
| Total Surface : |
595.64 μm2 |
| Total Volume : |
28.29 μm3 |
| Max Euclidean Distance : |
201.78 μm |
| Max Path Distance : |
211.09 μm |
| Max Branch Order : |
2 |
| Average Contraction : |
0.95 |
| Total Fragmentation : |
110 |
| Partition Asymmetry : |
0.4 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
70.99° |
| Average Bifurcation Angle Remote : |
85.28° |
| Fractal Dimension : |
1.01 |
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