8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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Details about selected cell
NeuroMorpho.Org ID :  NMO_181992
Cell Name :  20-MAX_WT-GLUTA-479-555-MAP2-EGFP-63X
Archive Name :  Siqueira_Guil
Species Name :  human
Strain :  Not applicable
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  2.5 months
Max Age :  2.5 months
Gender :  Not reported
Min Weight :  Not reported
Max Weight :  Not reported
Development :  fetus
Primary Brain Region :  mesencephalon
Secondary Brain Region :  ventral
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  glutamatergic
Tertiary Cell Class :  neural progenitor cell derived
Original Format :  Neuronstudio.swc
Experiment Protocol :  culture
Experimental Condition :  uc479 overexpression
Staining Method :  Farnesylated enhanced green fluorescent protein (eGFP-F)
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  63x
Reconstruction Method :  NeuronStudio
Date of Deposition :  2022-02-07
Date of Upload :  2022-04-06
Persistence Vector :  20-MAX_WT-GLUTA-479-555-MAP2-EGFP-63X.pvec
Note :  To improve circularization, a sequence of 35 nt upstream and downstream of introns was added. uc.479 were overexpressed by cloning into the EcoRI site of the vector pLVX-shRNA2.

Reference Article
Related Article Reference :  Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome.

Measurements
Soma Surface :  1156.58 μm2
Number of Stems :  11
Number of Bifurcations :  12
Number of Branches :  35
Overall Width :  39.93 μm
Overall Height :  56.9 μm
Overall Depth :  2.02 μm
Average Diameter :  1.02 μm
Total Length :  379.35 μm
Total Surface :  2795.92 μm2
Total Volume :  3999.86 μm3
Max Euclidean Distance :  45.47 μm
Max Path Distance :  52.97 μm
Max Branch Order :  7
Average Contraction :  0.88
Total Fragmentation :  363
Partition Asymmetry :  0.67
Average Rall's Ratio :  0.92
Average Bifurcation Angle Local :  102.53°
Average Bifurcation Angle Remote :  89.5°
Fractal Dimension :  1.09

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