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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_181946 |
| Cell Name : | 20-MAX_KO-GLUTA-478-555-MAP2-EGFP-63X |
| Archive Name : | Siqueira_Guil |
| Species Name : | human |
| Strain : | Not applicable |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 2.5 months
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| Max Age : | 2.5 months
|
| Gender : |
Not reported
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| Min Weight : | Not reported
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| Max Weight : | Not reported
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| Development : | fetus |
| Primary Brain Region : | mesencephalon |
| Secondary Brain Region : | ventral |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | glutamatergic |
| Tertiary Cell Class : | neural progenitor cell derived |
| Original Format : | Neuronstudio.swc |
| Experiment Protocol : | culture |
| Experimental Condition : | MECP2 knockout + uc478 overexpression |
| Staining Method : | Farnesylated enhanced green fluorescent protein (eGFP-F) |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 63x |
| Reconstruction Method : | NeuronStudio |
| Date of Deposition : | 2022-02-07 |
| Date of Upload : | 2022-04-06 |
| Persistence Vector : | 20-MAX_KO-GLUTA-478-555-MAP2-EGFP-63X.pvec |
| Note : | CRISPR-Cas9 technology was used for engineering MECP2 KO in human ReNCells VM. Mecp2 KO was confirmed by western blotting. MeCP2 was restored in human MeCP2 KO cells. To improve circularization, a sequence of 35 nt upstream and downstream of introns was added. uc.478 were overexpressed by cloning into the BamHI site of the vector pLVX-shRNA2. |
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| Reference Article |
| Related Article Reference : | Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome. |
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| Measurements |
| Soma Surface : |
839.67 μm2 |
| Number of Stems : |
6 |
| Number of Bifurcations : |
6 |
| Number of Branches : |
18 |
| Overall Width : |
26.83 μm |
| Overall Height : |
89.47 μm |
| Overall Depth : |
0.65 μm |
| Average Diameter : |
1.19 μm |
| Total Length : |
237.18 μm |
| Total Surface : |
2137.61 μm2 |
| Total Volume : |
3321.17 μm3 |
| Max Euclidean Distance : |
56.85 μm |
| Max Path Distance : |
59.75 μm |
| Max Branch Order : |
3 |
| Average Contraction : |
0.95 |
| Total Fragmentation : |
230 |
| Partition Asymmetry : |
0.33 |
| Average Rall's Ratio : |
0.63 |
| Average Bifurcation Angle Local : |
98.55° |
| Average Bifurcation Angle Remote : |
94.47° |
| Fractal Dimension : |
1.04 |
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