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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_283583 |
| Cell Name : | 17_85 |
| Archive Name : | Wang_Y |
| Species Name : | chicken |
| Strain : | White leghorn |
| Structural Domains : | Dendrites, Soma, No Axon |
| Physical Integrity : | Dendrites Moderate |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 15.0 days
|
| Max Age : | 15.0 days
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | brainstem |
| Secondary Brain Region : | cochlear nucleus |
| Tertiary Brain Region : | nucleus magnocellularis |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | magnocellular |
| Tertiary Cell Class : | Not reported |
| Original Format : | Neurolucida.dat |
| Experiment Protocol : | in vitro |
| Experimental Condition : | Fmr1 knockdown |
| Staining Method : | Alexa Fluor 568 |
| Slicing Direction : | coronal |
| Slice Thickness : | 300
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 60x |
| Reconstruction Method : | Neurolucida |
| Date of Deposition : | 2019-03-08 |
| Date of Upload : | 2024-07-17 |
| Persistence Vector : | 17_85.pvec |
| Note : | This reconstruction was obtained from an embryo that received in ovo electroporation at Hamburger and Hamilton (HH) stage 12 with Fmr1 ShRNAs. Individual plasmids of the vector system were concentrated at 4-5 picograms/picoliters and then mixed at an equal amount. The plasmid mixture tinted with fast green was injected into the lumen of neural tube at the rhombomere 5/6 level which contain NM neuron precursors. The administration was performed again every other day to maintain the expression before tissue dissection at the desired developmental stages. Nontransfected NM neurons located within 100 microns away from a transfected neuron were individually dye-filled using electroporation. |
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| Reference Article |
| Related Article Reference : | Postsynaptic FMRP Regulates Synaptogenesis In Vivo in the Developing Cochlear Nucleus. |
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| Measurements |
| Soma Surface : |
894.51 μm2 |
| Number of Stems : |
15 |
| Number of Bifurcations : |
16 |
| Number of Branches : |
47 |
| Overall Width : |
32.48 μm |
| Overall Height : |
30.53 μm |
| Overall Depth : |
0.91 μm |
| Average Diameter : |
0.13 μm |
| Total Length : |
401.75 μm |
| Total Surface : |
164.08 μm2 |
| Total Volume : |
5.33 μm3 |
| Max Euclidean Distance : |
24.09 μm |
| Max Path Distance : |
32.19 μm |
| Max Branch Order : |
3 |
| Average Contraction : |
0.9 |
| Total Fragmentation : |
144 |
| Partition Asymmetry : |
0.33 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
74.07° |
| Average Bifurcation Angle Remote : |
58.3° |
| Fractal Dimension : |
1.05 |
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