8.6.82 - Released: 2025-05-21 - Content: 275062 cells
Morphology File (Standardized)
Morphology File (Original)
Log File (Standardized)
Log File (Original)



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NeuroMorpho.Org ID :  NMO_181921
Cell Name :  14-WT-GLUTA-478-555-MAP2-EGFP-63X
Archive Name :  Siqueira_Guil
Species Name :  human
Strain :  Not applicable
Structural Domains :  Dendrites, Soma, No Axon
Physical Integrity :  Dendrites Complete
Morphological Attributes :  No Diameter, 2D, Angles
Min Age :  2.5 months
Max Age :  2.5 months
Gender :  Not reported
Min Weight :  Not reported
Max Weight :  Not reported
Development :  fetus
Primary Brain Region :  mesencephalon
Secondary Brain Region :  ventral
Tertiary Brain Region :  Not reported
Primary Cell Class :  principal cell
Secondary Cell Class :  glutamatergic
Tertiary Cell Class :  neural progenitor cell derived
Original Format :  Neuronstudio.swc
Experiment Protocol :  culture
Experimental Condition :  uc478 overexpression
Staining Method :  Farnesylated enhanced green fluorescent protein (eGFP-F)
Slicing Direction :  Not applicable
Slice Thickness :  Not applicable
Tissue Shrinkage :  Not reported
Objective Type :  oil
Magnification :  63x
Reconstruction Method :  NeuronStudio
Date of Deposition :  2022-02-07
Date of Upload :  2022-04-06
Persistence Vector :  14-WT-GLUTA-478-555-MAP2-EGFP-63X.pvec
Note :  To improve circularization, a sequence of 35 nt upstream and downstream of introns was added. uc.478 were overexpressed by cloning into the BamHI site of the vector pLVX-shRNA2.

Reference Article
Related Article Reference :  Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome.

Measurements
Soma Surface :  891.91 μm2
Number of Stems :  5
Number of Bifurcations :  4
Number of Branches :  13
Overall Width :  25.16 μm
Overall Height :  54.73 μm
Overall Depth :  0.96 μm
Average Diameter :  1.34 μm
Total Length :  157.28 μm
Total Surface :  1340.68 μm2
Total Volume :  1385.52 μm3
Max Euclidean Distance :  42.49 μm
Max Path Distance :  45.76 μm
Max Branch Order :  4
Average Contraction :  0.97
Total Fragmentation :  119
Partition Asymmetry :  0.75
Average Rall's Ratio :  1.09
Average Bifurcation Angle Local :  110.42°
Average Bifurcation Angle Remote :  109.6°
Fractal Dimension :  1.03

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