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Details about selected cell |
NeuroMorpho.Org ID : | NMO_146597 |
Cell Name : | 12mOrange_final |
Archive Name : | Firestein |
Species Name : | rat |
Strain : | Sprague-Dawley |
Structural Domains : | Dendrites, Soma, No Axon |
Physical Integrity : | Dendrites Complete |
Morphological Attributes : | No Diameter, 2D, Angles |
Min Age : | 18.0 days
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Max Age : | 18.0 days
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Gender : |
Male/Female
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Min Weight : | Not reported
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Max Weight : | Not reported
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Development : | embryonic |
Primary Brain Region : | hippocampus |
Secondary Brain Region : | CA3 |
Tertiary Brain Region : | Not reported |
Primary Cell Class : | principal cell |
Secondary Cell Class : | pyramidal |
Tertiary Cell Class : | Developing |
Original Format : | NeuronJ.swc |
Experiment Protocol : | culture |
Experimental Condition : | Carboxypeptidase E (CPE) overexpression |
Staining Method : | enhanced green fluorescent protein, immunostaining |
Slicing Direction : | Not applicable |
Slice Thickness : | Not applicable
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Tissue Shrinkage : |
Not reported
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Objective Type : | dry |
Magnification : | 20x |
Reconstruction Method : | NeuronJ |
Date of Deposition : | 2020-11-16 |
Date of Upload : | 2021-01-05 |
Persistence Vector : | 12mOrange_final.pvec |
Note : | Cotransfected with pCAG-mOrange and pEGFP-C1-CPE-C10 at DIV 7 using Lipofectamine LTX with Plus, fixed at DIV 10 with 4% PFA in PBS, and immunostained for GFP and MAP2. |
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Reference Article |
Related Article Reference : | Cortical Neuron Migration and Dendrite Morphology are Regulated by Carboxypeptidase E. |
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Measurements |
Soma Surface : |
10.53 μm2 |
Number of Stems : |
9 |
Number of Bifurcations : |
21 |
Number of Branches : |
51 |
Overall Width : |
10.78 μm |
Overall Height : |
16.78 μm |
Overall Depth : |
0 μm |
Average Diameter : |
0.3 μm |
Total Length : |
111.26 μm |
Total Surface : |
104.86 μm2 |
Total Volume : |
7.86 μm3 |
Max Euclidean Distance : |
10.61 μm |
Max Path Distance : |
11.62 μm |
Max Branch Order : |
5 |
Average Contraction : |
0.98 |
Total Fragmentation : |
227 |
Partition Asymmetry : |
0.52 |
Average Rall's Ratio : |
2 |
Average Bifurcation Angle Local : |
57.26° |
Average Bifurcation Angle Remote : |
48.52° |
Fractal Dimension : |
1.01 |
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