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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_254612 |
| Cell Name : | 118483R72hrs7 |
| Archive Name : | Morgan |
| Species Name : | mouse |
| Strain : | Rosa26-LSL-Bdnf-myc-IG/Pf4iCre |
| Structural Domains : | Dendrites, No Soma, No Axon |
| Physical Integrity : | Dendrites Complete |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 2.0 months
|
| Max Age : | 6.0 months
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| Gender : |
Male/Female
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| Min Weight : | 20 grams
|
| Max Weight : | 28 grams
|
| Development : | adult |
| Primary Brain Region : | retina |
| Secondary Brain Region : | right |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | ganglion |
| Tertiary Cell Class : | Not reported |
| Original Format : | Imaris.ims |
| Experiment Protocol : | ex vivo |
| Experimental Condition : | Retinal explant + ZEB85 |
| Staining Method : | DiOlistics |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | dry |
| Magnification : | 20x |
| Reconstruction Method : | Imaris |
| Date of Deposition : | 2022-11-09 |
| Date of Upload : | 2023-02-27 |
| Persistence Vector : | 118483R72hrs7.pvec |
| Note : | Mice culled by cervical dislocation. Retinas dissected immediately after death and transferred to a culture insert with media supplemented with a TrkB agonist (ZEB85) 50micrograms/mL. Incubated for 72hrs. Retinas then labelled using DiOlisitics-Tungsten particles coated with DiI/DiO fired from a gene gun. After labelling, retinas were fixed in PFA and imaged by confocal microscopy. |
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| Reference Article |
| Related Article Reference : | BDDF released from blood platelets prevents dendritic atrophy of lesioned adult CNS neurons |
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| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
2 |
| Number of Bifurcations : |
7 |
| Number of Branches : |
16 |
| Overall Width : |
17.66 μm |
| Overall Height : |
25.95 μm |
| Overall Depth : |
4.73 μm |
| Average Diameter : |
1.62 μm |
| Total Length : |
147.7 μm |
| Total Surface : |
740.63 μm2 |
| Total Volume : |
468.34 μm3 |
| Max Euclidean Distance : |
15.49 μm |
| Max Path Distance : |
24.47 μm |
| Max Branch Order : |
8 |
| Average Contraction : |
0.87 |
| Total Fragmentation : |
286 |
| Partition Asymmetry : |
0.88 |
| Average Rall's Ratio : |
1.35 |
| Average Bifurcation Angle Local : |
114.95° |
| Average Bifurcation Angle Remote : |
81.64° |
| Fractal Dimension : |
1.11 |
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