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| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_280453 |
| Cell Name : | 10_144 |
| Archive Name : | Celdran |
| Species Name : | rat |
| Strain : | Sprague-Dawley |
| Structural Domains : | Neurites, Soma |
| Physical Integrity : | Neurites Moderate |
| Morphological Attributes : | No Diameter, 2D, Angles |
| Min Age : | 16.0 days
|
| Max Age : | 19.0 days
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | embryonic |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | principal cell |
| Secondary Cell Class : | embryonic stem cell (ESC)-derived |
| Tertiary Cell Class : | Not reported |
| Original Format : | NeuronJ.ndf |
| Experiment Protocol : | culture |
| Experimental Condition : | pCAG-GFP expression + nanodiamonds (ND12) nioplexes |
| Staining Method : | green fluorescent protein |
| Slicing Direction : | Not applicable |
| Slice Thickness : | Not applicable
|
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 40x , 63x |
| Reconstruction Method : | NeuronJ |
| Date of Deposition : | 2023-09-08 |
| Date of Upload : | 2024-03-14 |
| Persistence Vector : | 10_144.pvec |
| Note : | Cells in Neurobasal medium were seeded and incubated in 24-well plates between 21-28 days in vitro (DIV) before transfecting. Nioplexes were obtained by incubating each type of niosome with each plasmid. Then, an appropriate volume of each plasmid was mixed and incubated for 30 min at room temperature with the corresponding volume of each niosome suspension (1 mg/mL cationic lipid) to obtain the respective nioplexes at cationic lipid:DNA ratio 8:1. |
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| Reference Article |
| Related Article Reference : | Assessment of Different Niosome Formulations for Optogenetic Applications: Morphological and Electrophysiological Effects. |
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| Measurements |
| Soma Surface : |
2061.87 μm2 |
| Number of Stems : |
6 |
| Number of Bifurcations : |
4 |
| Number of Branches : |
14 |
| Overall Width : |
106.77 μm |
| Overall Height : |
58.01 μm |
| Overall Depth : |
0.07 μm |
| Average Diameter : |
1 μm |
| Total Length : |
368.5 μm |
| Total Surface : |
1157.69 μm2 |
| Total Volume : |
289.42 μm3 |
| Max Euclidean Distance : |
70.06 μm |
| Max Path Distance : |
77.39 μm |
| Max Branch Order : |
4 |
| Average Contraction : |
0.97 |
| Total Fragmentation : |
64 |
| Partition Asymmetry : |
0.75 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
59.98° |
| Average Bifurcation Angle Remote : |
62.04° |
| Fractal Dimension : |
1.01 |
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