|
| Details about selected cell |
| NeuroMorpho.Org ID : | NMO_140530 |
| Cell Name : | 0293-S5-2-A2 |
| Archive Name : | Robel |
| Species Name : | mouse |
| Strain : | DTA floxxed//GLAST-creERT2 |
| Structural Domains : | Processes, No Soma |
| Physical Integrity : | Processes Moderate |
| Morphological Attributes : | No Diameter, 3D, Angles |
| Min Age : | 63.0 days
|
| Max Age : | 85.0 days
|
| Gender : |
Male/Female
|
| Min Weight : | Not reported
|
| Max Weight : | Not reported
|
| Development : | adult |
| Primary Brain Region : | neocortex |
| Secondary Brain Region : | Not reported |
| Tertiary Brain Region : | Not reported |
| Primary Cell Class : | Glia |
| Secondary Cell Class : | astrocyte |
| Tertiary Cell Class : | GFAP-positive |
| Original Format : | Simple Neurite Tracer.traces |
| Experiment Protocol : | in vivo |
| Experimental Condition : | astrocyte ablation (1 day post-tamoxifen) |
| Staining Method : | immunostaining |
| Slicing Direction : | horizontal |
| Slice Thickness : | 50
μm |
| Tissue Shrinkage : |
Not reported
|
| Objective Type : | oil |
| Magnification : | 60x |
| Reconstruction Method : | Simple Neurite Tracer |
| Date of Deposition : | 2020-06-08 |
| Date of Upload : | 2020-10-07 |
| Persistence Vector : | 0293-S5-2-A2.pvec |
| Note : | Floxxed Diptheria toxin fragment A + cre-recombinase expressed in front of GLAST promoter + single 330 mg/kg tamoxifen dose (40 mg/mL in 10% 200-proof ethanol and 90% corn oil shaking for 2h at 37C) at 8-12 weeks of age via oral gavage to induce recombination |
|
|
| Reference Article |
| Related Article Reference : | Astrocytes are necessary for blood-brain barrier maintenance in the adult mouse brain. |
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|
| Measurements |
| Soma Surface : |
N/A |
| Number of Stems : |
2 |
| Number of Bifurcations : |
93 |
| Number of Branches : |
188 |
| Overall Width : |
50.96 μm |
| Overall Height : |
47.59 μm |
| Overall Depth : |
6 μm |
| Average Diameter : |
0.25 μm |
| Total Length : |
952.51 μm |
| Total Surface : |
748.1 μm2 |
| Total Volume : |
46.76 μm3 |
| Max Euclidean Distance : |
34.66 μm |
| Max Path Distance : |
49.83 μm |
| Max Branch Order : |
21 |
| Average Contraction : |
0.84 |
| Total Fragmentation : |
3323 |
| Partition Asymmetry : |
0.68 |
| Average Rall's Ratio : |
2 |
| Average Bifurcation Angle Local : |
59.99° |
| Average Bifurcation Angle Remote : |
54.58° |
| Fractal Dimension : |
1.13 |
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